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<h1 id="firstHeading" class="firstHeading mw-first-heading"><span class="mw-page-title-main">TBS-Puffer</span></h1>
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<div id="mw-content-text" class="mw-body-content mw-content-ltr" lang="de" dir="ltr"><div class="mw-content-ltr mw-parser-output" lang="de" dir="ltr"><p><b>TBS-Puffer</b> (<span style="font-style:normal;font-weight:normal"><a href="Englische_Sprache" title="Englische Sprache">englisch</a></span> <span lang="en-Latn" style="font-style:italic"><i>Tris-buffered saline</i></span>, TBS) ist ein in der <a href="Biochemie" title="Biochemie">Biochemie</a> zur <a href="L%C3%B6sung_(Chemie)" title="Lösung (Chemie)">Lösung</a> von <a href="Protein" title="Protein">Proteinen</a> verwendeter <a href="Puffer_(Chemie)" title="Puffer (Chemie)">Puffer</a>.
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<div class="mw-heading mw-heading2"><h2 id="Eigenschaften">Eigenschaften</h2></div>
<p>TBS-Puffer wird zur Untersuchung der <a href="Adsorption" title="Adsorption">Bindung</a> und zur Lagerung von Proteinen sowie zur <a href="Immundetektion" class="mw-redirect" title="Immundetektion">Immundetektion</a> verwendet, darunter im <a href="Western_Blot" title="Western Blot">Western Blot</a>, im <a href="ELISA" class="mw-redirect" title="ELISA">ELISA</a>, im <a href="ELISPOT" class="mw-redirect" title="ELISPOT">ELISPOT</a>, in der <a href="Immunfluoreszenz" title="Immunfluoreszenz">Immunfluoreszenz</a> und in der <a href="Immunhistochemie" title="Immunhistochemie">Immunhistochemie</a>. TBS-Puffer besteht meistens aus 10 – 50 <a href="Molarit%C3%A4t" class="mw-redirect" title="Molarität">mM</a> <a href="TRIS" class="mw-redirect" title="TRIS">TRIS</a> als Puffersubstanz und 150 mM <a href="Natriumchlorid" title="Natriumchlorid">Natriumchlorid</a> für eine <a href="Tonizit%C3%A4t" title="Tonizität">physiologische</a> <a href="Ionenst%C3%A4rke" title="Ionenstärke">Ionenstärke</a>.<sup id="cite_ref-books-2xi9GlcLN2oC-99_1-0" class="reference"><a href="#cite_note-books-2xi9GlcLN2oC-99-1"><span class="cite-bracket">[</span>1<span class="cite-bracket">]</span></a></sup><sup id="cite_ref-books-3DNMdJwCo6YC-473_2-0" class="reference"><a href="#cite_note-books-3DNMdJwCo6YC-473-2"><span class="cite-bracket">[</span>2<span class="cite-bracket">]</span></a></sup> Der <a href="PH-Wert" title="PH-Wert">pH-Wert</a> wird je nach Protokoll mit verdünnter <a href="Salzs%C3%A4ure" title="Salzsäure">Salzsäure</a> auf 7,2 bis 7,6 eingestellt.<sup id="cite_ref-books-2xi9GlcLN2oC-99_1-1" class="reference"><a href="#cite_note-books-2xi9GlcLN2oC-99-1"><span class="cite-bracket">[</span>1<span class="cite-bracket">]</span></a></sup><sup id="cite_ref-books-MeqjqC92eSgC-411_3-0" class="reference"><a href="#cite_note-books-MeqjqC92eSgC-411-3"><span class="cite-bracket">[</span>3<span class="cite-bracket">]</span></a></sup> Der pH-Wert nimmt pro Kelvin um 0,03 ab. Alternativ zum TBS-Puffer werden unter anderem <a href="Good-Puffer" title="Good-Puffer">Good-Puffer</a> verwendet. TBS-Puffer mit <a href="Polysorbate" title="Polysorbate">Tween 20</a> wird als <a href="TBS-T-Puffer" title="TBS-T-Puffer">TBS-T-Puffer</a> bezeichnet.
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<div class="mw-heading mw-heading2"><h2 id="Einzelnachweise">Einzelnachweise</h2></div>
<ol class="references">
<li id="cite_note-books-2xi9GlcLN2oC-99-1"><span class="mw-cite-backlink">↑ <sup><a href="#cite_ref-books-2xi9GlcLN2oC-99_1-0">a</a></sup> <sup><a href="#cite_ref-books-2xi9GlcLN2oC-99_1-1">b</a></sup></span> <span class="reference-text">Academic Press: <i>Antibodies in Cell Biology.</i> Academic Press, 1993, ISBN 978-0-080-85935-4, S. 99.</span>
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<li id="cite_note-books-3DNMdJwCo6YC-473-2"><span class="mw-cite-backlink"><a href="#cite_ref-books-3DNMdJwCo6YC-473_2-0">↑</a></span> <span class="reference-text">M. A. Hayat: <i>Handbook of Immunohistochemistry and in Situ Hybridization of Human Carcinomas.</i> Academic Press, 2005, ISBN 978-0-080-49520-0, S. 473.</span>
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<li id="cite_note-books-MeqjqC92eSgC-411-3"><span class="mw-cite-backlink"><a href="#cite_ref-books-MeqjqC92eSgC-411_3-0">↑</a></span> <span class="reference-text">John Pound: <i>Immunochemical Protocols.</i> Springer Science & Business Media, 1998, ISBN 978-1-592-59257-9, S. 411.</span>
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Dieser Artikel wurde von <a class="external text" title="Zuletzt bearbeitet am 2021-03-18" href="https://de.wikipedia.org/wiki/?title=TBS-Puffer&oldid=209927868">Wikipedia</a> herausgegeben. Der Text ist unter <a class="external text" href="https://creativecommons.org/licenses/by-sa/4.0/deed.de">Creative Commons Attribution-Share Alike 4.0</a> verfügbar, sofern nicht anders angegeben. Für die Mediendateien können zusätzliche Bedingungen gelten.
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